Coding
YtvA
Part:BBa_K143037
Designed by: James Chappell Group: iGEM08_Imperial_College (2008-09-15)
YtvA Blue Light Receptor for B.subtilis
YtvA is a blue light receptor endogenously found in B.subtilis. Activation of the YtvA light receptor activates the mild stress response pathway, accumulating in sigma B activation. Previous research has shown that the sigma B response to blue light light exposure is enhanced by the over expression of the YtvA receptor 1. This part can be over expressed YtvA to enhance the transcriptional output of sigma B promoters in response to blue light.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 406
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 258
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 157
Illegal SapI site found at 475
References
<biblio>
- 1 pmid=17575448
</biblio>
Modifications in Sequence : iGEM21_IISER-Tirupati_India
BBa_K3889026 is a modified version specific to our project's assembly. Mutated Bsa1 and HindIII Sites to make an assembly compatible part.
[edit]
Categories
Parameters
//chassis/prokaryote/Bsubtilis
//cds/membrane/receptor
//function/sensor/light
//cds/membrane/receptor
//function/sensor/light
None |